Imbrenda 1
The effect of Temperature on Carbon dioxide Production in Cellular Respiration of Acheta domesticus
Introduction
Cellular respiration is the metabolic process where cells acquire energy by breaking down nutrient rich molecules that are produced by photosynthesis. The reactants of this metabolic process are glucose and oxygen. Its products consist of water, carbon dioxide, and adenosine triphosphate (ATP). Body temperature and efficiency of cellular respiration has a relationship in ectotherms, such as the common house cricket, or Acheta domesticus. This species is known for evolving in response to its thermal environment. Ectotherms must use environmental energy and behavioral adaptations to regulate their body temperatures since they can’t on their own. Once their internal body temperature is warm, they can metabolize foods and produce the energy they need. In this experiment, we will be measuring the amount of carbon dioxide produced at varying temperatures in order to determine the rate of cellular respiration.
Methods
Carbon dioxide sensors were used with a LoggerLite software setup. Make sure to calibrate the sensors and set them to 0-10,000 parts per million. Then a gas sensor is connected to a miniquest module which will record data it collects onto our computer via a USB port. Respiration chambers and three adult crickets were placed in each chamber and then were weighed. The experimental group was placed in chambers at room temperature, a cold temperature, and then a warm temperature. The crickets were measured while they acclimated for three minutes at each temperature to get an initial temperature. After a carbon dioxide gas sensor is placed inside the chambers containing the cricket and the carbon dioxide concentration was collected for three minutes. The coldest temperature was placed in an ice bath and the temperature was collect after the temperature reached 10 degrees below room temperature. Then repeat these steps for the warmer temperature once the temperature had reached 10 degrees above room temperature. Mean carbon dioxide produced was calculated for each experimental group over three minutes. Then a single factor ANOVA test was performed to analyze our collected data.
Results
Thee mean temperature change for the room temperature was 21.83 degrees Celcius The mean temperature for the colder temperature was 9.92 degrees Celcius. The mean temperature for the warmer temperature was 33.58 degrees Celcius. There was an increase in average temperature for the warmer temperature and a decrease in average temperature for the colder temperature. A single factor ANOVA was used to calculate a p-value of 2.12*10^-32.
This chart shows the experiments (n=36) mean carbon dioxide production and temperature change for the crickets. The crickets at room temperature had a standard deviation of + .4. The crickets at a colder temperature had a standard deviation of + .61 and the crickets at the warmer temperature had a standard deviation of + .76
Discussion
Crickets will have a higher cellular respiration rate in warmer temperatures and a lower respiration rate in colder temperatures. This is because crickets are Ectotherms and ectotherms rely on their surrounding energy of the environment in order to undergo metabolic processes and remain at homeostasis. This is because all cells require a form of energy, usually ATP. ATP is broken down by cells so that the energy can be released. Temperature plays a clear role in the efficiency of this process. Although in simpler forms of life such as plants or invertebrates, there is little to no change seen in metabolic rates due to change in temperature. Our collected data provided evidence that the crickets will exhibit more carbon dioxide produced in higher temperatures than in colder temperatures. This is due to the temperature altering the crickets cellular activity thus interfering with their cellular respiration.
Literature Cited
http://www.nms.org/getmedia/befb3bfe-6549-4527-8083-0b6ee68934a2/Cricket-Respiration.pdf.aspx National Math + Science initiative, Dallas, Texas Vernier 2007
https://undergradsciencejournals.okstate.edu/index.php/jibi/article/view/3082
Kaden Kennedy, Jonathan McCaslin, Amber Grant, Matthew Liao 2016
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2131248/
Geneva A. Daland and Raphael Isaacs 1927(Revised)
Framing Letter:
Strengths:
In this lab report, I showed strengths through my Introduction and Methods. In the introduction, I clearly stated the background information needed for any reader to understand what was being done in the lab. It also depicts my hypothesis of the results from the lab and why the lab was relevant compared to other work. Next, in the methods section, all materials required for the lab were presented as well as the steps in which to use them. This is a strength because it shows exactly how I did my experiment and gives the required information for the reader to repeat the experiment themselves.
Weaknesses:
Weaknesses of this lab report appeared in my Results and Discussion section. In my results section, I just spewed a bunch of statistics from the data I had collected onto the paper without making any real transitions. Although you are not to explain any reasoning to your data in this section it still needs to sound readable without sounding like bullet points. Then in my discussion section, I was not as strong as I could’ve been in explaining why my collected data made sense or not. Also, I needed to incorporate my sources much more efficiently and effectively to really let the reader get a better understand of what I am trying to say.
